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Research (Published
online : 16-05-2013)
8. Ameliorative potential of Tephrosia purpurea
extract against arsenic induced toxicity in wistar rats -
Ravuri Halley Gora, Sushma Lalita Baxla, Priscilla Kerketta, Reetu Toppo,
Naveen Kumar and Birendra kumar Roy
Vet World. 2013; 6(8): 493-496
doi:
10.5455/vetworld.2013.493-496
Abstract
Aim: The present
investigation has been conducted to evaluate the protective activity of
Tephrosia purpurea extract (TPE) against arsenic induced toxicity.
Materials and Methods: For this study, twenty four wistar albino rats were
taken. Control group, group – I rats were given sodium arsenite @ 10 mg/kg and
group – II rats were treated with TPE @ 500 mg/kg along with sodium arsenite @
10 mg/kg (daily oral for 28 days). On 29th day animals were slaughtered and
various parameters were determined. Serum biomarkers, haematological parameter
analysis and histomorphological examination are carried out with estimation of
arsenic concentration in tissues. Results: Oral administration of
sodium arsenite @ 10 mg/kg for 28 days resulted in a significant decrease in
Hb%, TEC and TLC, significant increase of serum glucose, cholesterol, calcium
and significant increase in arsenic accumulation in tissues. Histopathological
results of intestine revealed haemorrhagic enteritis along with loss of villi.
Treatment with Tephrosia purpurea @ 500 mg/kg significantly decreased the
elevated glucose, LDH levels, along with significant increase haematological
levels towards normal. There was reduced haemorrhagic enteritis and presence
of intact villi, as compared to arsenic treated group. But there was no
significant difference in serum calcium, serum cholesterol and arsenic
concentration in tissues, when compared with arsenic treated group.
Conclusion: The study conclude that supplementation of TPE (500 mg/kg)
daily oral for 28 days has shown protection against arsenic induced toxicity
by its protective effect.
Keywords: arsenic, haematology, rats, serum
bio-markers, Tephrosia purpurea, toxicity
